文章摘要
吕兴1,2,许惠琴1,2*,吕高虹1,2,吴云皓1,2,陈玉萍1,2,沈红胜1,2,戴国英1,2,徐康1,2.AGEs/RAGE/SphK1信号通路介导马钱苷抗AGEs损伤GMCs的机制研究[J].南京中医药大学学报(自然科学版),2017,33(4):382-385.
AGEs/RAGE/SphK1信号通路介导马钱苷抗AGEs损伤GMCs的机制研究
Study on Mechanism of Loganin Against AGEs Induced GMCs Injury via AGEs/RAGE/SphK1 Pathway
投稿时间:2017-01-15  修订日期:2017-04-18
DOI:
中文关键词: 山茱萸  马钱苷  晚期糖基化终末产物  肾小球系膜细胞  糖尿病肾病
英文关键词: Cornus officinalis  loganin  advanced glycation end products  glomerulus mesangial cell  diabetic nephropathy
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作者单位
吕兴1,2,许惠琴1,2*,吕高虹1,2,吴云皓1,2,陈玉萍1,2,沈红胜1,2,戴国英1,2,徐康1,2 1.南京中医药大学药学院江苏 南京 2100232.江苏省中药药效与安全性评价重点实验室江苏 南京 210023 
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中文摘要:
      目的 探讨山茱萸中有效成分马钱苷在缓解晚期糖基化终末产物(AGEs)诱导肾小球系膜细胞(GMCs)损伤的机制。方法 体外培养GMCs,分为空白对照组,模型组(AGEs:200mg/L),马钱苷0.1、1、10μmol/L浓度组。检测细胞增殖率、细胞FN及COL-Ⅳ分泌水平,观察细胞微观结构变化,测定RAGE、SphK1、S1P和TGF-β蛋白表达量。结果 马钱苷可有效抑制AGEs诱导的GMCs增殖,减少FN及COL-Ⅳ分泌,缓解细胞微观结构病变,下调RAGE、SphK1、S1P和TGF-β蛋白表达。结论 马钱苷通过下调AGEs/RAGE/SphK1信号通路,缓解AGEs诱导的GMCs损伤,从而改善糖尿病肾病(DN)。
英文摘要:
      OBJECTIVE To study the mechanism of loganin, the effective componet of Cornus officinalis, on protecting the glomerulus mesangial cell (GMCs) injured by advanced glycation end products (AGEs). METHODS GMCs were divided into control, model (AGEs: 200mg/L), loganin (0.1, 1, 10μmol/L) groups. The cell proliferation, FN and COL-Ⅳ secretion, microstructure lesions, RAGE, SphK1, S1P and TGF-β protein expressions were measured. RESULTS AGEs induced cell proliferation, FN and COL-Ⅳ secretions, microstructure lesions, RAGE, SphK1, S1P and TGF-β protein expressions were suppressed by loganin. CONCLUSION Loganin can improve the AGEs induced GMCs injury by regulating the AGEs/RAGE/SphK1 signal pathway, and then ameliorate the diabetic nephropathy.
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